A CRISPR screen targeting PI3K effectors identifies RASA3 as a negative regulator of LFA-1–mediated adhesion in T cells

by Klaus Okkenhaug
in Publications
on 20/07/2022

New collaborative work with Pam Schwartzberg’s lab at the NIH, led by Kristoffer Johansen who shared his time between our labs during his Wellcome Trust-funded PhD and as part of the NIH-OxCam programme.

The work is the result of a CRISPR/Cas9 screen looking for PIP3 effector proteins that regulate T cell adhesion via LFA-1. RASA3 was among the most interesting targets and was investigated further using conditional knockout mouse models.

Kristoffer, Pam and Klaus at the BiochemSoc PI3K meeting in Buxton, 2019.

Full text of the article here: https://doi.org/10.1126/scisignal.abl9169

Kristoffer also wrote and excellent tweetorial you can read here:

Our study describing a CRISPR screen of PI3Kinase-mediated regulation of ICAM-1 binding by the major T cell integrin, LFA-1, is out!

Check below for my attempt at a T cell signaling #tweetorial.https://t.co/fmJ6HZXoxU pic.twitter.com/Nqcf8hJ2n8
— Kristoffer H. J. (@KrisHaurum) July 19, 2022

A CRISPR screen targeting PI3K effectors identifies RASA3 as a negative regulator of LFA-1–mediated adhesion in T cells

A CRISPR screen targeting PI3K effectors identifies RASA3 as a negative regulator of LFA-1–mediated adhesion in T cells

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